Adenovirus Fiber Shaft Contains a Trimerization Element That Supports Peptide Fusion for Targeted Gene Delivery

Adenovirus fiber shaft contains a trimerization element that supports peptide fusion for targeted gene delivery.

Adenoviral (Ad) vectors have been widely used in human gene therapy clinical trials. However, their application has frequently been restricted by the unfavorable expression of cell surface receptors critical for Ad infection. Infections by Ad2 and Ad5 are largely regulated by the elongated fiber protein that mediates its attachment to a cell surface receptor, coxsackie and adenovirus receptor (...

design and development of a series of recombinant fusion vectors for targeted cancer gene delivery

Enhanced gene transfer activity of peptide-targeted gene-delivery vectors.

We have evaluated the capacity of the cell-binding heptapeptide SIGYPLP to enhance transgene expression using non-viral and viral gene delivery vectors. Targeted polyplex based vectors showed good levels of DNA uptake in freshly isolated human umbilical vein endothelial cells (HUVECs) compared to untargeted controls, whilst displaying only modest increases in reporter gene activity. The targete...

Human enteric adenovirus type 41 (Tak) contains a second fiber protein gene.

DNA sequencing of the human enteric adenovirus type 41 Tak position 83.1% to the AccI site at map position 87 .1%. Bases (1) genome upstream from the fiber protein gene (2) revealed 1161 to 1402 overlap the sequence published earlier by us (2). an open reading frame of 387 amino acids coding for a 41.4K protein. The first 42 amino acids of this protein show a high RFFERENCES degree of homology ...

A mouse model for adenovirus gene delivery.

The cellular attachment receptor for adenovirus (Ad), Coxsackie adenovirus receptor (CAR), required for delivery of Ad into primary cells, is not present on all cell types, thus restricting Ad-gene delivery systems. To circumvent this constrain, a transgenic mouse has been generated that expresses a truncated human CAR in all tissues analyzed. These mice allowed efficient in vitro infections at...